IL-1 beta antibody (Internal Region)

Catalog No. ABIN6262623

Product Details anti-IL-1 beta Antibody

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: IL1 beta Antibody detects endogenous levels of total IL1 beta.

Predicted Reactivity: Horse,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human IL1 beta, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-IL-1 beta antibody (ABIN6262623)

Zhu, Chen, Dai, Zheng, Jiang, Xu, Sheng, Guo, Dan, Liang, Li: "Chronic lead exposure enhances the sympathoexcitatory response associated with P2X4 receptor in rat stellate ganglia." in: Environmental toxicology, Vol. 33, Issue 6, pp. 631-639, (2018) (PubMed).

Hu, Cheng, Huang, Cai, Lian, Wang, Gao: "Effects of Xin-Ji-Er-Kang on heart failure induced by myocardial infarction: Role of inflammation, oxidative stress and endothelial dysfunction." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 42, pp. 245-257, (2018) (PubMed).

Sheng, Wang, Guo, Xu, Jiang, Zheng, Xu, Zhang, Che, Liang, Zhu, Li: "Effects of Baicalin on Diabetic Cardiac Autonomic Neuropathy Mediated by the P2Y12 Receptor in Rat Stellate Ganglia." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 46, Issue 3, pp. 986-998, (2018) (PubMed).

Zhu, Xu, Lv, He, Ye, Wang, Hu: "miR-199b-5p Regulates Immune-Mediated Allograft Rejection after Lung Transplantation Through the GSK3β and NF-κB Pathways." in: Inflammation, Vol. 41, Issue 4, pp. 1524-1535, (2018) (PubMed).

Zhu, Shi, Lv, Li, Pan, Chen, Zhao, Yan, Chen, Loake, Niu, Zeng, Xu: "Loss of NLRP3 Function Alleviates Murine Hepatic Graft-versus-Host Disease." in: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, (2018) (PubMed).

Guo, Sheng, Dan, Xu, Zhang, Ji, Wang, Xu, Che, Li, Liang, Li: "Involvement of P2Y12 receptor of stellate ganglion in diabetic cardiovascular autonomic neuropathy." in: Purinergic signalling, (2018) (PubMed).

Feng, Gao, Cui, Li, Li, Cui, Cui: "Neuroprotective Effects of Resatorvid Against Traumatic Brain Injury in Rat: Involvement of Neuronal Autophagy and TLR4 Signaling Pathway." in: Cellular and molecular neurobiology, Vol. 37, Issue 1, pp. 155-168, (2017) (PubMed).

Ding, Xu, Wang, Zhang: "Rotenone Attenuates Renal Injury in Aldosterone-Infused Rats by Inhibiting Oxidative Stress, Mitochondrial Dysfunction, and Inflammasome Activation." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 3136-43, (2016) (PubMed).

Feng, Cui, Gao, Li, Li, Jiang, Tian, Wang, Cui, Cui: "Resveratrol attenuates neuronal autophagy and inflammatory injury by inhibiting the TLR4/NF-κB signaling pathway in experimental traumatic brain injury." in: International journal of molecular medicine, Vol. 37, Issue 4, pp. 921-30, (2016) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B (IL1B Products)

Synonyms: IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody

Background:

Description: Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

Gene: IL1B

Molecular Weight: 30 kDa(precursor),17kDa(mature)

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome