STAT1 antibody

Catalog No. ABIN6265311

There are 2+ publications for this product available. The Rabbit Polyclonal anti-STAT1 antibody is suitable to detect STAT1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IP, IF and ICC.

Quick Overview for STAT1 antibody (ABIN6265311)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-STAT1 Antibody

Specificity: STAT1 Antibody detects endogenous levels of total STAT1

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human STAT1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IP, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-STAT1 antibody (ABIN6265311)

Liu, Wu, Zhu, Li, Liu: "IRG1 increases MHC class I level in macrophages through STAT-TAP1 axis depending on NADPH oxidase mediated reactive oxygen species." in: International immunopharmacology, Vol. 48, pp. 76-83, (2018) (PubMed).

Chen, Zhou, Liu, Huang, Liu, Kang, Chen, Guo, Zhu, Sun: "Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway." in: Oncology reports, Vol. 39, Issue 3, pp. 1081-1089, (2018) (PubMed).

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT1

Background:

Description: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Gene: STAT1

Molecular Weight: 84kDa

Gene ID: 6772

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events