UBE2N antibody (Internal Region)

Catalog No. ABIN6265853

Product Details anti-UBE2N Antibody

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: UBE2N Antibody detects endogenous levels of total UBE2N.

Predicted Reactivity: Bovine,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human UBE2N, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: UBE2N (UBE2N Products)

Synonyms: UBC13 antibody, UbcH-ben antibody, UbcH13 antibody, 1500026J17Rik antibody, AL022654 antibody, BB101821 antibody, ube2n antibody, wu:fb11b03 antibody, wu:fc08b06 antibody, zgc:55726 antibody, ubc13 antibody, ubch-ben antibody, UBE2N antibody, ube2nl antibody, zgc:63901 antibody, ubiquitin conjugating enzyme E2 N antibody, ubiquitin-conjugating enzyme E2N antibody, ubiquitin conjugating enzyme E2 N S homeolog antibody, ubiquitin-conjugating enzyme E2Na antibody, ubiquitin-conjugating enzyme E2Nb antibody, UBE2N antibody, Ube2n antibody, ube2n.S antibody, ube2na antibody, LOC100533434 antibody, ube2n antibody, ube2nb antibody

Background:

Description: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.

Gene: UBE2N

Molecular Weight: 17 kDa

Gene ID: 7334

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway