PPARG antibody (AA 50-130)

Catalog No. ABIN6284221

Product Details anti-PPARG Antibody

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Binding Specificity: AA 50-130

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PPAR-γ is a protein encoded by the PPARG gene which is approximately 57,6 kDa. PPAR-γ is localised to the cytoplasm and nucleus. It is involved in regulation of lipid metabolism, gene expression, the nuclear receptor transcription pathway and AMP-activated protein kinase signalling. This protein falls under the peroxisome proliferator-activated receptor subfamily. It is a nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once logand activated, the nuclear receptor binds to DNA specific PPAR response elements and modulates the transcription of its target genes, such as acyl-CoA oxidase. PPAR-γ has the highest expression in adipose tissue. Mutations in the PPARG gene may result in diabetes mellitus and is a cardiovascular disease risk factor. STJ95201 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PPAR-γ protein.

Specificity: PPAR-γ Polyclonal Antibody detects endogenous levels of PPAR-γ protein.

Characteristics: Rabbit polyclonal to PPAR-γ.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PPAR-gamma around the non-phosphorylation site of S112.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Comment:

Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPAR-gamma (PPARG Products)

Synonyms: PPAR gamma antibody, PPARg2 antibody, PPARG antibody, Nr1c3 antibody, PPAR-gamma antibody, PPAR-gamma2 antibody, PPARgamma antibody, PPARgamma2 antibody, NR1C3 antibody, CIMT1 antibody, GLM1 antibody, PPARG1 antibody, PPARG2 antibody, xPPAR-gamma antibody, PPAR-GAMMA antibody, PPARGAMMA antibody, peroxisome proliferator-activated receptor gamma antibody, peroxisome proliferator activated receptor gamma antibody, peroxisome proliferator activated receptor gamma L homeolog antibody, PPARG antibody, Pparg antibody, pparg.L antibody

Molecular Weight: 57 kDa

Gene ID: 5468

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation