HIF3A antibody (Internal Region)

Catalog No. ABIN6286917

Product Details anti-HIF3A Antibody

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal to HIF-3α.

Specificity: HIF-3α Polyclonal Antibody detects endogenous levels of HIF-3α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human HIF-3alpha.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ELISA 1:20000

Comment:

Expressed in vascular cells (at protein level) . Expressed in kidney . Expressed in lung epithelial cells . Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta) . Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney . Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells . Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues . Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines . Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas . Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen . Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Alternative Name: HIF-3alpha (HIF3A Products)

Synonyms: HIF-3A antibody, IPAS antibody, MOP7 antibody, PASD7 antibody, bHLHe17 antibody, Ipas antibody, NEPAS antibody, Mop7 antibody, HIF3A antibody, hypoxia inducible factor 3 alpha subunit antibody, hypoxia inducible factor 3, alpha subunit antibody, HIF3A antibody, Hif3a antibody

Molecular Weight: 72 kDa

UniProt: Q9Y2N7

Pathways: Warburg Effect