JNK antibody (pThr183)

Catalog No. ABIN6288138

Product Details anti-JNK Antibody

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Binding Specificity: AA 120-200, pThr183

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: JNK1/2/3 is a protein encoded by the MAPK8 gene which is approximately 48,2 kDa. JNK1/2/3 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the IL-2 pathway, TNFR1 pathway, toll-like receptor signalling pathways and the 4-1BB pathway. It acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, therefore mediating immediate-early gene expression in response to cell stimuli. JNK1/2/3 is expressed in the nervous system, liver, blood, heart and lung. Mutations in the MAPK8 gene may result in fatty liver disease and renal fibrosis. STJ90316 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous JNK1/2/3 protein which only binds about T183 when T183 is phosphorylated.

Specificity: Phospho-JNK1/2/3 (T183) Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at T183.

Characteristics: Rabbit polyclonal to Phospho-JNK1/2/3 (T183).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of T183.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK1/2/3 (MAPK8 Products)

Synonyms: JNK antibody, JNK-46 antibody, JNK1 antibody, JNK1A2 antibody, JNK21B1/2 antibody, PRKM8 antibody, SAPK1 antibody, SAPK1c antibody, AI849689 antibody, Prkm8 antibody, BSK antibody, BSK/DJNK antibody, Bsk antibody, CG5680 antibody, D-JNK antibody, D-junk antibody, DBSK/JNK antibody, DJNK antibody, DJNK/bsk antibody, Dmel\\CG5680 antibody, JNK/SAPK antibody, Jnk antibody, Junk antibody, SAPKa antibody, c-Jun antibody, dJNK antibody, jnk antibody, T10F20.15 antibody, jnk1 antibody, sapk1 antibody, mapk8 antibody, zgc:112379 antibody, JNKb antibody, mapk8b antibody, mitogen-activated protein kinase 8 antibody, Stress-activated protein kinase jnk-1 antibody, basket antibody, Protein kinase superfamily protein antibody, mitogen-activated protein kinase 8 L homeolog antibody, mitogen-activated protein kinase 8b antibody, mitogen-activated protein kinase 8B-like antibody, MAPK8 antibody, jnk-1 antibody, Mapk8 antibody, bsk antibody, ATMPK8 antibody, mapk8.L antibody, mapk8b antibody, LOC109094263 antibody

Molecular Weight: 48 kDa

Gene ID: 5599

UniProt: P45983

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins