NTRK3 antibody (pTyr516)

Catalog No. ABIN6288355

Product Details anti-NTRK3 Antibody

Target: NTRK3 (Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3))

Binding Specificity: AA 460-540, pTyr516

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NTRK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Trk C is a protein encoded by the NTRK3 gene which is approximately 94,4 kDa. Trk C is localised to the cell membrane and is involved in apoptotic pathways in synovial fibroblasts, GPCR pathway, ERK signalling and apoptosis pathways. This protein falls under the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Trk C is widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain. Mutations in the NTRK3 gene are associated with susceptibility to congenital heart defects and differentiated thyroid carcinoma. STJ90960 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.This primary antibody specifically binds to endogenous Trk C protein which only binds about Y516 when Y516 is phosphorylated.

Specificity: Phospho-Trk C (Y516) Polyclonal Antibody detects endogenous levels of Trk C protein only when phosphorylated at Y516.

Characteristics: Rabbit polyclonal to Phospho-Trk C (Y516).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Trk C around the phosphorylation site of Y516.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Comment:

Widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain than in fetal brain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for NTRK3

Target: NTRK3 (Neurotrophic tyrosine Kinase, Receptor, Type 3 (NTRK3))

Alternative Name: Trk C (NTRK3 Products)

Synonyms: TRKC antibody, gp145(trkC) antibody, trkC antibody, AW125844 antibody, Ntrk3_tv3 antibody, TrkC antibody, neurotrophic receptor tyrosine kinase 3 antibody, neurotrophic tyrosine kinase, receptor, type 3 antibody, NTRK3 antibody, Ntrk3 antibody

Molecular Weight: 160 kDa

Gene ID: 4916

UniProt: Q16288

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, Regulation of Cell Size