MAPT antibody (Microtubule-Associated Protein tau) (pSer396)

Details for Product anti-MAPT Antibody No. ABIN6652127
Antigen
  • DDPAC
  • FTDP-17
  • MAPTL
  • MSTD
  • MTBT1
  • MTBT2
  • PPND
  • TAU
  • AI413597
  • AW045860
  • Mtapt
  • Tau
  • RNPTAU
  • pTau
  • tau
  • xtp
  • MAPT
  • PHF-tau
  • slc6a6
  • taut
  • wu:fc26e12
  • microtubule associated protein tau
  • microtubule-associated protein tau
  • microtubule associated protein tau S homeolog
  • Microtubule-associated protein tau
  • solute carrier family 6 (neurotransmitter transporter), member 6b
  • MAPT
  • Mapt
  • mapt.S
  • LOC5580230
  • CpipJ_CPIJ013260
  • tau
  • slc6a6b
Alternatives
anti-Human MAPT antibody for Western Blotting
Epitope
pSer396
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
1225
802
728
98
39
27
26
20
16
11
8
5
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3
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Host
Rabbit
1165
100
15
7
Clonality
Polyclonal
Conjugate
This MAPT antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1056
450
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Options
Immunogen The antiserum was produced against synthesized phosphopeptide derived from Human Tau around the phosphorylation site of Serine 396 (Y-K-Sp-P-V).
Purification Immunoaffinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others MAPT products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name MAPT / tau (MAPT Antibody Abstract)
Background Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau
Gene ID 9606
UniProt P10636
Pathways MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size
Application Notes Western blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-Microtubule-Associated Protein tau (MAPT) (pSer396) antibody (ABIN6652127) anti-Microtubule-Associated Protein tau (MAPT) (pSer396) antibody
 image for anti-Microtubule-Associated Protein tau (MAPT) (pSer396) antibody (ABIN6652127) anti-Microtubule-Associated Protein tau (MAPT) (pSer396) antibody (Image 2)
Product cited in: Thysiadis, Mpousis, Avramidis, Katsamakas, Balomenos, Remelli, Efthimiopoulos, Sarli: "Discovery of novel phenoxazinone derivatives as DKK1/LRP6 interaction inhibitors: Synthesis, biological evaluation and structure-activity relationships." in: Bioorganic & medicinal chemistry, Vol. 24, Issue 5, pp. 1014-22, 2016 (PubMed).

Mpousis, Thysiadis, Avramidis, Katsamakas, Efthimiopoulos, Sarli: "Synthesis and evaluation of gallocyanine dyes as potential agents for the treatment of Alzheimer's disease and related neurodegenerative tauopathies." in: European journal of medicinal chemistry, Vol. 108, pp. 28-38, 2016 (PubMed).

Eritja, Santacana, Maiques, Gonzalez-Tallada, Dolcet, Matias-Guiu: "Modeling glands with PTEN deficient cells and microscopic methods for assessing PTEN loss: endometrial cancer as a model." in: Methods (San Diego, Calif.), Vol. 77-78, pp. 31-40, 2015 (PubMed).

Jiang, Wang, Wang, Hawke, Zheng, Li, Zhou, Majumder, Bi, Liu, Huang, Lu: "PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells." in: Nature communications, Vol. 5, pp. 5566, 2015 (PubMed).

Bockmeyer, Kern, Forstmeier, Lovric, Modde, Agustian, Steffens, Birschmann, Traeder, Dämmrich, Schwarz, Kreipe, Bröcker, Becker: "Arteriolar vascular smooth muscle cell differentiation in benign nephrosclerosis." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 27, Issue 9, pp. 3493-501, 2013 (PubMed).

Gui, Han, Zhang, Liang, Wang, Xuan, Yu, Shang: "Dimerization of ZIP promotes its transcriptional repressive function and biological activity." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 886-95, 2012 (PubMed).

Lu, Berglund, Larsson, Höög, Farnebo, Bränström: "Calmodulin and calmodulin-dependent protein kinase II inhibit hormone secretion in human parathyroid adenoma." in: The Journal of endocrinology, Vol. 208, Issue 1, pp. 31-9, 2011 (PubMed).

Ishiguro, Ando, Maeda, Watanabe, Goto: "Cutting edge: tubulin ? functions as an adaptor in NFAT-importin ? interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 2710-3, 2011 (PubMed).

Godfrey, Anderson, Papanastasiou, Takeuchi, Boshoff: "Inhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA." in: Blood, Vol. 105, Issue 6, pp. 2510-8, 2005 (PubMed).

Dempsey, Sailor, Bowen, Türeyen, Vemuganti: "Stroke-induced progenitor cell proliferation in adult spontaneously hypertensive rat brain: effect of exogenous IGF-1 and GDNF." in: Journal of neurochemistry, Vol. 87, Issue 3, pp. 586-97, 2003 (PubMed).

Watanabe, Sugaya, Atkins, Aquilino, Yang, Borris, Brady, Blauvelt: "Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen prolongs the life span of primary human umbilical vein endothelial cells." in: Journal of virology, Vol. 77, Issue 11, pp. 6188-96, 2003 (PubMed).

Background publications Puig, Rey, Ferrer: "Individual and regional variations of phospho-tau species in progressive supranuclear palsy." in: Acta neuropathologica, Vol. 110, Issue 3, pp. 261-8, 2005 (PubMed).

DeGiorgis, Jaffe, Moreira, Carlotti, Leite, Pant, Dosemeci: "Phosphoproteomic analysis of synaptosomes from human cerebral cortex." in: Journal of proteome research, Vol. 4, Issue 2, pp. 306-15, 2005 (PubMed).

Alonso, Mederlyova, Novak, Grundke-Iqbal, Iqbal: "Promotion of hyperphosphorylation by frontotemporal dementia tau mutations." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34873-81, 2004 (PubMed).

Kyriakis, Banerjee, Nikolakaki, Dai, Rubie, Ahmad, Avruch, Woodgett: "The stress-activated protein kinase subfamily of c-Jun kinases." in: Nature, Vol. 369, Issue 6476, pp. 156-60, 1994 (PubMed).

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