SCN9A antibody (C-Term)

Catalog No. ABIN6656787

Product Details anti-SCN9A Antibody

Target: SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCN9A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-NAV1.7 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with NAV1.7 from Human, Rat, and Mouse based on 100% homology with the immunizing sequence. No cross-reactivity against other Nav channels. Cross-reactivity with NAV1.7 from other sources has not been determined. Channels and Transporters research.

Immunogen:

Immunogen: Nav1.7 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to the cytoplasmic C-terminus region of human Nav1.7.

Immunogen Type: Recombinant Protein

Clone: S68-6

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-NAV1.7 Antibody is suitable for use in WB, IP, and IF microscopy. Expect a band approximately ~230 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for SCN9A

Target: SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))

Alternative Name: NAV1.7 (SCN9A Products)

Synonyms: SCN9A antibody, DKFZp459G084 antibody, Nav1.7 antibody, PN1 antibody, mKIAA4197 antibody, Scn2a antibody, ETHA antibody, FEB3B antibody, GEFSP7 antibody, NE-NA antibody, NENA antibody, SFNP antibody, sodium channel, voltage-gated, type IX, alpha subunit antibody, sodium voltage-gated channel alpha subunit 9 antibody, sodium channel protein type 9 subunit alpha antibody, sodium channel, voltage-gated, type IX, alpha antibody, SCN9A antibody, LOC100016648 antibody, Scn9a antibody

Background:

Synonyms: ETHA, hNE Na, NENA, PN1, SCN9A, voltage gated sodium channel subunit alpha Nav1.7, peripheral sodium channel 1, neuroendocrine sodium channel, Sodium channel protein type 9 subunit alpha

Background: Nav1.7 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.7 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.7 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals.

Gene Name: Scn9a

Gene ID: 6335

NCBI Accession: NP_002968

UniProt: Q15858