c-MYC antibody (C-Term)

Catalog No. ABIN6657589

Supplier Product No.: 100-401-224

The Rabbit Polyclonal anti-c-MYC antibody has been validated for WB and ELISA. It is suitable to detect c-MYC in samples from Mouse.

Quick Overview for c-MYC antibody (C-Term) (ABIN6657589)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-c-MYC Antibody

Binding Specificity: C-Term

Supplier Product No.: 100-401-224

Supplier: Rockland

Purpose: c-Myc Antibody

Cross-Reactivity (Details): Anti-c-myc is highly specific for c-myc and shows no cross-reaction with N-myc or L-myc proteins.

Purification: This antiserum is directed against human c-myc and is useful in determining its presence in various assays.

Sterility: Sterile filtered

Immunogen: This whole rabbit serum was prepared by repeated immunizations with c-myc peptide corresponding to amino acids KHKLEQLRNSGA which map to the last 12 amino acids of the mouse

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 1:500- 1:2,000

Other: User Optimized

Comment:

Anti-c-myc has been tested by western blot. This antibody reacts with human and mouse c-myc protein, which is a nuclear protein with a molecular weight of approximately 66 kDa.  This antiserum detects c-myc by immunoblotting assays in both overexpressed and endogenous systems.   Reactivity in other immunoassays has not yet been fully determined although perinuclear and cytoplasmic staining has been reported.  The recommended positive controls are SK-BR-3 cells (adenocarcinoma, breast, malignant pleural effusion, human).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: c-Myc

Background:

Synonyms: rabbit anti-c-Myc Antibody, Myc proto-oncogene protein antibody, Proto-oncogene c-Myc antibody, Transcription factor p64 antibody, Class E basic helix-loop-helix protein 39 antibody

Background: c-myc is involved in the control of cell proliferation and differentiation at the transition from S --> G₁ phase for a normal cell.  Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain), 2 (k light chain), or 22 (l light chain) is described in Burkitt's lymphoma and other B-cell lympho-proliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.  Abnormal expression can take many forms including transduction, insertional activation, translocation, and amplification.

Gene Name: Myc, c-myc

Gene ID: 17869

NCBI Accession: NP_001170823

UniProt: P01108

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect