CDK5 antibody (AA 15-100)

Catalog No. ABIN670101

This anti-CDK5 antibody is a Rabbit Polyclonal antibody detecting CDK5 in WB, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for CDK5 antibody (AA 15-100) (ABIN670101)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CDK5 Antibody

Binding Specificity: AA 15-100

Specificity: This antibody will detect both isoform variants of this protein. Due to similar amino acid composition of this protein to CDK3 in human, there is a chance that this antibody will react with CDK3 in human, based on an 83 % sequence similarity.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CDK5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CDK5 antibody (ABIN670101)

Cao, Jia, Wei, Liu, Liu, Li: "Traditional Chinese Medicine Huannao Yicong Decoction Extract Decreases Tau Hyperphosphorylation in the Brain of Alzheimer's Disease Model Rats Induced by Aβ1-42." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 6840432, (2016) (PubMed).

Yin, Qi, Ren, Wang, Jiang, Feng, Cui: "Roscovitine treatment caused impairment of fertilizing ability in mice." in: Toxicology letters, Vol. 237, Issue 3, pp. 200-9, (2015) (PubMed).

Li, Wang, Guo, Huang, Zhao, Zhu, Ma, Liang, Zhang, Huang, Wan: "Protective effect of Xingnaojia formulation on rats with brain and liver damage caused by chronic alcoholism." in: Experimental and therapeutic medicine, Vol. 10, Issue 5, pp. 1643-1652, (2015) (PubMed).

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background:

Synonyms: PSSALRE, Cyclin-dependent-like kinase 5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, CDK5, CDKN5

Background: CDK5 is serine/threonine kinase involved in synaptic regulation and neuronal development, phosphorylates synaptic protein Pctaire1, regulates acetylcholine receptor expression.CDK5 is a member of the cyclindependent kinase family of serine/threonine kinases. It is present in numerous mammalian tissues including kidney, testes, and ovary. Its activity is detected almost exclusively in brain extracts. Neuronal and muscle cells contain the highest amount of this protein. Similar to other Cdks, monomeric Cdk5 displays no enzymatic activity, but Cdk5 is not activated by cyclins. Instead, the p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 interacts with D1 and D3 type G1 cyclins and can phosphorylate histone H1, TAU, MAP2 and NF-H and NF-M. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.

Gene ID: 1020

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis