SPI1 antibody (C-Term)

Catalog No. ABIN6740536

Product Details anti-SPI1 Antibody

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Monkey, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human SPI1 / PU.1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Chicken, Zebrafish (100%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SPI1 (P17947, NP_003111). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Trout, Catfish, Salmon, Zebrafish (100%), Xenopus, Sablefish (85%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1 / PU.1 (SPI1 Products)

Synonyms: OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody

Background: Name/Gene ID: SPI1

Synonyms: SPI1, 31 kDa-transforming protein, 31 kDa transforming protein, PU.1, SFPI1, SPI-1, SPI-1 proto-oncogene, SPI-A, Transcription factor PU.1

Gene ID: 6688

NCBI Accession: NP_003111

UniProt: P17947

Pathways: Stem Cell Maintenance