ERCC8 antibody (AA 72-121)

Catalog No. ABIN6741407

Product Details anti-ERCC8 Antibody

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Binding Specificity: AA 72-121

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC8 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human ERCC8

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Bovine, Rabbit, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human ERCC8 (B2RB64, NP_000073). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Galago (92%), Marmoset, Bat (90%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ERCC8

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Alternative Name: CSA / ERCC8 (ERCC8 Products)

Synonyms: zgc:103550 antibody, CKN1 antibody, CSA antibody, UVSS2 antibody, Ckn1 antibody, 2410022P04Rik antibody, 2810431L23Rik antibody, 4631412O06Rik antibody, B130065P18Rik antibody, Csa antibody, excision repair cross-complementation group 8 antibody, ERCC excision repair 8, CSA ubiquitin ligase complex subunit antibody, excision repaiross-complementing rodent repair deficiency, complementation group 8 antibody, ercc8 antibody, ERCC8 antibody, Ercc8 antibody

Background: Name/Gene ID: ERCC8

Synonyms: ERCC8, CKN1, UVSS2, CSA

Gene ID: 1161

NCBI Accession: NP_000073

UniProt: Q13216

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus