ERCC8 antibody (AA 72-121)

Catalog No. ABIN6741407

This Rabbit Polyclonal antibody specifically detects ERCC8 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Horse and Cow.

Quick Overview for ERCC8 antibody (AA 72-121) (ABIN6741407)

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERCC8 Antibody

Binding Specificity: AA 72-121

Specificity: Human ERCC8

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Bovine, Rabbit, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human ERCC8 (B2RB64, NP_000073). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Galago (92%), Marmoset, Bat (90%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ERCC8

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Alternative Name: CSA / ERCC8

Background: Name/Gene ID: ERCC8

Synonyms: ERCC8, CKN1, UVSS2, CSA

Gene ID: 1161

NCBI Accession: NP_000073

UniProt: Q13216

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus