CD36 antibody

Catalog No. ABIN6936675

Product Details anti-CD36 Antibody

Target: CD36

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD36 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Specificity: Recognizes a protein of 80 kDa-90 kDa, identified as CD36 (Workshop IV, Code P-26). Its epitope maps between aa155-183. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4 % of Japanese and East Asia population lack CD36. This MAb blocks adhesion of P. falciparum parasitized red blood cells to CD36 and strongly inhibits collagen-induced platelet aggregation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leukocytes

Clone: 185-1G2

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: HEL or U937 cells. Platelets, monocytes, macrophages, microvascular endothelial cells in a tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD36

Target: CD36

Alternative Name: CD36 (CD36 Products)

Synonyms: BDPLT10 antibody, CHDS7 antibody, FAT antibody, GP3B antibody, GP4 antibody, GPIV antibody, PASIV antibody, SCARB3 antibody, Fat antibody, Scarb3 antibody, GPIIIB antibody, PAS-4 antibody, zgc:92513 antibody, CD36 molecule antibody, CD36 molecule (thrombospondin receptor) antibody, CD36 antibody, Cd36 antibody, cd36 antibody

Background: Adipocyte membrane protein, CHDS7, Collagen receptor, platelet, fatty acid translocase (FAT), Fatty acid transport protein, glycoprotein iiib (GP IIIb), PAS IV, PAS-4, pas4 protein, platelet collagen receptor, Platelet glycoprotein 4, scarb3, Scavenger receptor class B member 3, thrombospondin receptor,CD36 (Platelet & Microvessel Marker)
Cellular localisation: Cell surface

Molecular Weight: 80-90kDa

Gene ID: 948, 120949

UniProt: P16671

Pathways: TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins