CKB antibody
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- Target See all CKB Antibodies
- CKB (Creatine Kinase, Brain (CKB))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CKB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human full-length CKB protein
- Clone
- CPTC-CKB-2
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product CKB Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: SH-SY5Y or HEK-293 cells. Human cerebellum.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- CKB (Creatine Kinase, Brain (CKB))
- Alternative Name
- CKB (CKB Products)
- Synonyms
- B-CK antibody, CKBB antibody, b-ck antibody, ckbb antibody, CKB antibody, Bck antibody, Ck-3 antibody, Ck3 antibody, Ckbb antibody, bck antibody, ckb antibody, cb594 antibody, wu:fj36f10 antibody, wu:fj37d11 antibody, Ckbr antibody, mg:cb02h12 antibody, wu:fb62d04 antibody, wu:fc14g11 antibody, zgc:154095 antibody, zgc:92079 antibody, creatine kinase B antibody, creatine kinase, brain L homeolog antibody, creatine kinase B-type antibody, creatine kinase, brain antibody, creatine kinase, brain b antibody, creatine kinase, brain a antibody, CKB antibody, ckb.L antibody, Ckb antibody, ckb antibody, ckbb antibody, ckba antibody
- Background
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BCK, Brain creatine kinase, Ckb, Creatine kinase B chain, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine phosphokinase BB, Epididymis luminal protein 211, Epididymis secretory protein Li 29, HEL 211,Creatine Phosphokinase-BB (CK-BB)
Cellular localisation: Cytoplasm - Molecular Weight
- 42kDa
- Gene ID
- 1152, 173724
- UniProt
- P12277
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