EGLN1 antibody (AA 1-24)
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- Target See all EGLN1 Antibodies
- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
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Binding Specificity
- AA 1-24
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EGLN1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.
- Cross-Reactivity (Details)
- Human. Immunogen sequence has 83 % identity with Mouse.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Residues 1-24 of PHD2
- Clone
- 366G-76-3
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product EGLN1 Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF7 cells. Brain or adrenal gland.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
- Alternative Name
- EGLN1 (EGLN1 Products)
- Synonyms
- EGLN1 antibody, egln2 antibody, phd2 antibody, zgc:92314 antibody, AI503754 antibody, C1orf12 antibody, HIF-PH2 antibody, HPH-2 antibody, Hif-p4h-2 antibody, ORF13 antibody, Phd2 antibody, SM-20 antibody, PHD-2 antibody, PHD2 antibody, ECYT3 antibody, HIFPH2 antibody, HPH2 antibody, SM20 antibody, ZMYND6 antibody, egl nine homolog 1 (C. elegans) antibody, egl-9 family hypoxia inducible factor 1 antibody, egl-9 family hypoxia-inducible factor 1 S homeolog antibody, egl-9 family hypoxia-inducible factor 1b antibody, egl-9 family hypoxia-inducible factor 1 antibody, EGLN1 antibody, egln1.S antibody, egln1b antibody, Egln1 antibody
- Background
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Chromosome 1 Open Reading Frame 12 (C1ORF12), DKFZp761F179, ECYT3, Egl 9 family hypoxia inducible factor 1, Egl nine homolog 1, Egln1, HIF Prolyl Hydroxylase 2 (HIF PH2), HIFPH2, HPH2, Hypoxia inducible factor prolyl hydroxylase 2, ORF13, P4H2, PhD2, PNAS 118, PNAS 137, Prolyl hydroxylase domain-containing protein 2, SM20, Zinc finger MYND domain containing protein 6 (ZMYND6),EGLN1 / PHD2
Cellular localisation: Cytoplasm. - Molecular Weight
- 46kDa
- Gene ID
- 54583, 444450
- UniProt
- Q9GZT9
- Pathways
- cAMP Metabolic Process, Warburg Effect
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