Frataxin antibody (AA 57-210)

Catalog No. ABIN6937289

Product Details anti-Frataxin Antibody

Target: Frataxin (FXN)

Binding Specificity: AA 57-210

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Frataxin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Frataxin is a nuclear-encoded mitochondrial protein implicated in Friedreich's ataxia (FRDA), a human autosomal recessive neurodegenerative disease. Lack of Frataxin causes iron to accumulate in the mitochondrial matrix suggesting that Frataxin is involved in mitochondrial iron homeostasis and possibly in iron transport. Frataxin has an alpha-beta fold consisting of two helices flanking an antiparallel beta sheet.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human Frataxin (FXN) protein (around aa 57-210) (exact sequence is proprietary)

Clone: FXN-2124

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, Panc-2, HL-60 or HEK293 cells. Pancreas and Liver.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Frataxin

Target: Frataxin (FXN)

Alternative Name: FXN (FXN Products)

Synonyms: zgc:158218 antibody, LOC100284365 antibody, BcDNA:AT09528 antibody, CG8971 antibody, DFH antibody, Dfh antibody, Dmel\\CG8971 antibody, X25 antibody, dfh antibody, CyaY antibody, FA antibody, FARR antibody, FRDA antibody, RGD1565754 antibody, Frda antibody, frataxin antibody, fxn antibody, LOC100284365 antibody, TVAG_182150 antibody, fh antibody, FTX1 antibody, FXN antibody, Fxn antibody

Background: CyaY, d-FXN, FARR, Frataxin mature form, Frataxin (81-210), FRDA, Friedreich ataxia protein, Fxn, i-FXN, m56-FXN, m78-FXN, m81-FXN, X25,Frataxin (Marker of Friedreich Ataxia)
Cellular localisation: Cytoplasmic (Mitochondria)

Molecular Weight: 14kDa

Gene ID: 2395, 20685

UniProt: Q16595

Pathways: Transition Metal Ion Homeostasis, Regulation of Cell Size