HLA-DRA antibody

Catalog No. ABIN6937412

Product Details anti-HLA-DRA Antibody

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected. '

Cross-Reactivity (Details): Human. Monkey.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Spleen cells of a patient with hairy cell leukemia (Daudi cells)

Clone: IPO-10

Isotype: IgG3 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Alternative Name: HLA-DRA (HLA-DRA Products)

Synonyms: HLA-DRA1 antibody, MLRW antibody, DR-alpha antibody, HLA-DRA antibody, Mamu-DRA1 antibody, major histocompatibility complex, class II, DR alpha L homeolog antibody, major histocompatibility complex, class II, DR alpha antibody, hla-dra.L antibody, HLA-DRA antibody, MAMU-DRA antibody, BOLA-DRA antibody

Background: DR alpha chain, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DRA, HLA DRA1, HLA-DRA, HLADRA1, Major histocompatibility complex class II DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA,HLA-DRA (MHC II)
Cellular localisation: Cell surface

Molecular Weight: ~36kDa

Gene ID: 3122, 520048

UniProt: P01903

Pathways: TCR Signaling, CXCR4-mediated Signaling Events