SOX2 antibody (AA 176-305)

Catalog No. ABIN6938410

Product Details anti-SOX2 Antibody

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Binding Specificity: AA 176-305

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Cross-Reactivity (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Clone: SOX2-1791

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, NCCIT cells. Lung or Cervicalcarcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2 (SOX2 Products)

Synonyms: ANOP3 antibody, MCOPS3 antibody, RGD1565646 antibody, Sox-2 antibody, lcc antibody, ysb antibody, cb236 antibody, wu:fb83g04 antibody, wu:fc14d07 antibody, zgc:65860 antibody, zgc:77389 antibody, SRY-box2 antibody, Xsox-2 antibody, anop3 antibody, mcops3 antibody, sox-2 antibody, SRY-box 2 antibody, SRY box 2 antibody, SRY (sex determining region Y)-box 2 antibody, SRY-box 2 S homeolog antibody, SOX2 antibody, Sox2 antibody, sox2 antibody, sox2.S antibody

Background: ANOP3, Delta EF2a,MCOPS3 (Microphthalmia Syndromic type 3),SOX-2, SRY (sex determining region Y) box 2, SRY related HMG box 2, Transcription factor SOX-2, ysb,SOX2 (Embryonic Stem CellMarker)
Cellular localisation: Nuclear

Molecular Weight: 34kDa

Gene ID: 6657, 518438

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis