SPI1 antibody (AA 16-170)

Catalog No. ABIN6938424

Product Details anti-SPI1 Antibody

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Binding Specificity: AA 16-170

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)

Clone: PU1-2146

Isotype: IgG2b kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL),Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, K562 or THP-1 cells. Lymph Node, Spleen, Hodgkin s Lymphoma, Colon Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1 (SPI1 Products)

Synonyms: OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody

Background: Transcription Factor spi1, 31 kDa Transforming Protein, Hematopoietic Transcription Factor PU.1, SFPI1, SPI1, SPIA, Spleen focus forming virus (SFFV) proviral integration oncogene spi1,PU.1 (SPI-1) (B-Cell Marker)
Cellular localisation: Nuclear

Molecular Weight: 40kDa

Gene ID: 6688, 502511

UniProt: P17947

Pathways: Stem Cell Maintenance