CD25 antibody
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- Target See all CD25 (IL2RA) Antibodies
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD25 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)
- Specificity
- Recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 Molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant full-length human IL2RA protein
- Clone
- IL2RA-423
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product IL2RA Primary Antibody
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- Application Notes
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Positive Control: PHA-stimulated human lymphocytes. Human lymph nodes and tonsils.
Known Application: Functional Studies (Order Ab without Azide), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Alternative Name
- IL2RA (IL2RA Products)
- Synonyms
- CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
- Molecular Weight
- 55kDa
- Gene ID
- 3559
- UniProt
- P01589
- Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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