LIG1 antibody

Catalog No. ABIN6935350

Product Details anti-LIG1 Antibody

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purification: Purified by Protein A/G

Immunogen: Full-length native cow LIG1 protein

Clone: 1A9

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.

Known Application: Western Blot (0.5-1 μg/mL), ELISA, Immunoprecipitation,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: LIG1 (LIG1 Products)

Synonyms: ATLIG1 antibody, DNA ligase 1 antibody, T6D22.23 antibody, ligI antibody, AL033288 antibody, LigI antibody, lig1 antibody, wu:fc54a11 antibody, DNA ligase 1 antibody, DNA ligase 1, putative antibody, ligase I, DNA, ATP-dependent L homeolog antibody, ligase I, DNA, ATP-dependent antibody, LIG1 antibody, PB000674.02.0 antibody, PTRG_06243 antibody, PKH_140260 antibody, lig1.L antibody, Lig1 antibody, lig-1 antibody, lig1 antibody

Background: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Molecular Weight: 133kDa

Gene ID: 3978

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA