Recombinant MUC1 antibody

Catalog No. ABIN6940097

Product Details anti-MUC1 Antibody

Target: MUC1 (Mucin 1 (MUC1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

Purification: Purified by Protein A/G

Immunogen: Recombinant human MUC1 protein

Clone: RMUC1-960

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1 (MUC1 Products)

Synonyms: CA 15-3 antibody, CD227 antibody, EMA antibody, H23AG antibody, KL-6 antibody, MAM6 antibody, MCKD1 antibody, MUC-1 antibody, MUC-1/SEC antibody, MUC-1/X antibody, MUC1/ZD antibody, PEM antibody, PEMT antibody, PUM antibody, Muc-1 antibody, mucin 1, cell surface associated antibody, mucin 1, transmembrane antibody, MUC1 antibody, Muc1 antibody

Molecular Weight: 265-400kDa

Gene ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling