RBP4 antibody

Catalog No. ABIN6934582

Product Details anti-RBP4 Antibody

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reactivity: Human, Mouse, Rat, Rabbit, Monkey, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Specificity: Recognizes a protein of 21 kDa-25 kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.

Purification: Purified by Protein A/G

Immunogen: Retinol binding protein Purified from human plasma

Clone: SPM442

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Liver or placenta.

Known Application: Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Alternative Name: RBP (RBP4 Products)

Synonyms: RDCCAS antibody, RBP antibody, RNBP antibody, rbp4 antibody, MGC54038 antibody, srbp antibody, PRBP antibody, RBPA antibody, Rbp-4 antibody, fb23c12 antibody, rbp antibody, wu:fb23c12 antibody, wu:fb58d04 antibody, wu:fb72b04 antibody, zgc:86911 antibody, retinol binding protein 4 antibody, renin binding protein antibody, retinol binding protein 4 S homeolog antibody, retinol binding protein 4 A, plasma antibody, retinol binding protein 4, plasma antibody, retinol binding protein 4 L homeolog antibody, RBP4 antibody, RENBP antibody, rbp4.S antibody, rbp4 antibody, Rbp4 antibody, RBP4A antibody, rbp4.L antibody

Molecular Weight: 21-25kDa

Gene ID: 5947, 5948, 5950

UniProt: P02753, P09455, P50120

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response