TLE1 antibody (AA 175-338)
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- Target See all TLE1 Antibodies
- TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))
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Binding Specificity
- AA 175-338
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TLE1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant fragment (aa 175-338) of human TLE1 (exact sequence is proprietary)
- Clone
- TLE1-2085
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product TLE1 Primary Antibody
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- Application Notes
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Positive Control: HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas.
Known Application: Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- TLE1 (Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))
- Alternative Name
- TLE1 (TLE1 Products)
- Synonyms
- ESG antibody, ESG1 antibody, GRG1 antibody, C230057C06Rik antibody, Estm14 antibody, Grg1 antibody, Tle4l antibody, TLE1 antibody, transducin like enhancer of split 1 antibody, transducin-like enhancer protein 1 antibody, transducin-like enhancer of split 1 antibody, transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) antibody, TLE1 antibody, Smp_150080 antibody, Tle1 antibody, LOC100354911 antibody, LOC100524475 antibody, LOC100061341 antibody
- Background
- Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that isencoded by theTLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
- Molecular Weight
- 83kDa
- Gene ID
- 7088
- UniProt
- Q04724
- Pathways
- WNT Signaling, SARS-CoV-2 Protein Interactome
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