Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human, Mouse, Rat, Pig, Cow, Cat, Rabbit, Sheep
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Staining Methods (StM)
- Specificity
- This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
- Immunogen
- Human thymic nuclear extract
- Clone
- LN-6
- Isotype
- IgM kappa
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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Positive Control: Jurkat cells, Sarcomas, Melanomas.
Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Vimentin (VIM)
- Alternative Name
- VIM (VIM Products)
- Synonyms
- CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
- Molecular Weight
- 57-60kDa
- Gene ID
- 7431
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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