Recombinant GFP antibody (Alexa Fluor 647)
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- Target See all GFP Antibodies
- GFP (Green Fluorescent Protein (GFP))
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
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Reactivity
- Aequorea victoria
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- This GFP antibody is conjugated to Alexa Fluor 647
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Application
- Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- Purpose
- Camelid sdAb anti-GFP conjugated with Alexa647, Clones 1H1/1B2
- Specificity
- Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.
- Cross-Reactivity (Details)
- Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
- Characteristics
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- Purification
- Produced in: E.coli
- Clone
- 1H1-1B2
- Labeling Ratio
- A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.
- Top Product
- Discover our top product GFP Primary Antibody
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- Application Notes
- Recommended dilution 1:500
- Comment
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A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable.
- Concentration
- 5 μM
- Buffer
- Lyophilized from PBS pH 7.4.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light!
- Storage
- -20 °C,-80 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
- Expiry Date
- 6 months
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- Target
- GFP (Green Fluorescent Protein (GFP))
- Alternative Name
- GFP (GFP Products)
- Synonyms
- green fluorescent protein antibody, gfp antibody
- Target Type
- Viral Protein
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