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5-Hydroxymethylcytosine antibody

5-hmC Reactivity: Human, Mouse DB, ICC, MeDIP, IF, IHC Host: Mouse Monoclonal 59-1 unconjugated
Catalog No. ABIN6971329
  • Target See all 5-Hydroxymethylcytosine (5-hmC) products
    5-Hydroxymethylcytosine (5-hmC)
    Reactivity
    Human, Mouse
    Host
    • 12
    • 4
    Mouse
    Clonality
    • 10
    • 6
    Monoclonal
    Conjugate
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    This 5-Hydroxymethylcytosine antibody is un-conjugated
    Application
    • 9
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dot Blot (DB), Immunocytochemistry (ICC), Methylated DNA Immunoprecipitation (MeDIP), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Characteristics
    In addition to this monoclonal, We offer two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version and a purified IgG version .All are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, we recommend either this monoclonal or the purified IgG polyclonal . The whole serum version is very high titre and should be used carefully (0.1 - 0.5 µL per IP) as not to generate high non-specific background. The whole serum version has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version is likely to work in this application as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine. 5-Hydroxymethylcytosine (5-hmC) antibody (mAb) (Clone 59.1) was raised in a Mouse host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Methyl DNA Immunoprecipitation, it has been shown to react with Human and Mouse samples, but the sequence is not species specific so it should react with a wide range of sample types.
    Purification
    Protein G Chromatography
    Immunogen
    This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.
    Clone
    59-1
    Isotype
    IgG2a
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target
    5-Hydroxymethylcytosine (5-hmC)
    Abstract
    5-hmC Products
    Target Type
    Chemical
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