ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
This polyclonal antibody has no cross response to IKKa or IKKy.
Purification
IKK beta Antibody is affinity chromatography purified via peptide column.
Immunogen
IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid. The immunogen is located within amino acids 650 - 700 of IKK beta.
IKBKB
Reactivity: Human
ELISA, WB, IHC, IF
Host: Rabbit
Monoclonal
3E1
unconjugated
Recombinant Antibody
Application Notes
IKK beta antibody can be used for detection of IKK beta by Western blot at 0.5 μ,g/mL. A 87 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
IKK beta Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
IKK beta antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
IKK beta Antibody: Nuclear factor kappa B (NF-κ,B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ,B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κ,B is associated with Iκ,B proteins in the cell cytoplasm, which inhibit NF-κ,B activity. The long-sought Iκ,B kinase (IKK), which phosphorylates Iκ,B, and mediates Iκ,B degradation and NF-κ,B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα, and IKKβ,). IKKα, and IKKβ, interact with each other and both are essential for NF-κ,B activation. IKKβ, phosphorylates both Iκ,B-alpha and Iκ,B-beta. IKKβ, is expressed in variety of human tissues.