DFFB antibody (AA 190-240)

Catalog No. ABIN6990306

Product Details anti-DFFB Antibody

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Binding Specificity: AA 190-240

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Purification: DFF40 Antibody is affinity chromatography purified via peptide column.

Immunogen: DFF40 antibody was raised against a 16 amino acid peptide near the center of human DFF40. The immunogen is located within amino acids 190 - 240 of DFF40.

Isotype: IgG

Application Details

Application Notes: DFF40 antibody can be used for detection of DFF40 /CAD by Western blot at 0.5 - 2 μ,g/mL. dilution. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DFF40 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DFF40 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for DFFB

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Alternative Name: DFF40 (DFFB Products)

Synonyms: CAD antibody, CPAN antibody, DFF-40 antibody, DFF2 antibody, DFF40 antibody, LOC100223514 antibody, 40kDa antibody, 5730477D02Rik antibody, Didff antibody, Cad antibody, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase antibody, DNA fragmentation factor subunit beta antibody, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) antibody, DNA fragmentation factor, beta subunit antibody, CAD antibody, DFFB antibody, dffb antibody, LOC100223514 antibody, Dffb antibody

Background: DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Molecular Weight: Predicted: 37 kDa

Observed: 38 kDa

Gene ID: 13368

NCBI Accession: NP_004393

UniProt: O54788

Pathways: Apoptosis, Caspase Cascade in Apoptosis