TIRAP Antibody is Ion exchange chromatography purified.
Immunogen
TIRAP antibody was raised against a peptide corresponding to amino acids near the middle of human TIRAP. The immunogen is located within amino acids 80 - 130 of TIRAP.
TIRAP antibody can be used for detection of TIRAP by Western blot at 4 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 20 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
TIRAP Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
TIRAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
TIRAP
(Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))
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Background
TIRAP Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-κ,B and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.