TIRAP antibody (C-Term)

Catalog No. ABIN6990430

Product Details anti-TIRAP Antibody

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIRAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: At least three isoforms of TIRAP are know to exist, this antibody detects all three isoforms.

Purification: TIRAP Antibody is affinity chromatography purified via peptide column.

Immunogen: TIRAP antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of mouse TIRAP. The immunogen is located within the last 50 amino acids of TIRAP.

Isotype: IgG

Application Details

Application Notes: TIRAP antibody can be used for detection of TIRAP by Western blot at 1 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TIRAP Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TIRAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TIRAP

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Alternative Name: TIRAP (TIRAP Products)

Synonyms: mal antibody, wyatt antibody, BACTS1 antibody, Mal antibody, MyD88-2 antibody, AA407980 antibody, C130027E04Rik antibody, Tlr4ap antibody, Wyatt antibody, toll-interleukin 1 receptor (TIR) domain containing adaptor protein antibody, TIR domain containing adaptor protein antibody, toll-interleukin 1 receptor (TIR) domain containing adaptor protein L homeolog antibody, toll-interleukin 1 receptor (TIR) domain-containing adaptor protein antibody, tirap antibody, TIRAP antibody, tirap.L antibody, Tirap antibody

Background: TIRAP Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kappaB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.

Molecular Weight: Predicted: 24, 26 kDa

Observed: 24 kDa

Gene ID: 114609

NCBI Accession: NP_473437

UniProt: P58753

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades