CASP5 antibody (AA 170-220)

Catalog No. ABIN6990517

Product Details anti-CASP5 Antibody

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Binding Specificity: AA 170-220

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Depending on cell lines or tissues used, other cleavage products may be observed.

Purification: Caspase-5 Antibody is affinity chromatography purified via peptide column.

Immunogen: Caspase-5 antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5. The immunogen is located within amino acids 170 - 220 of Caspase-5.

Isotype: IgG

Application Details

Application Notes: Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Caspase-5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Caspase-5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CASP5

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Alternative Name: Caspase-5 (CASP5 Products)

Synonyms: ICE(rel)III antibody, ICEREL-III antibody, ICH-3 antibody, CASP5 antibody, caspase 5 antibody, caspase-5 antibody, CASP5 antibody, LOC100437308 antibody

Background: Caspase-5 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β, and IL-18. Caspase-5 can interact with caspase-1, both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β,. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.

Gene ID: 838

UniProt: P51878

Pathways: Inflammasome