Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
Specificity
Detects isoforms Mcl-1L and Mcl-1S
Purification
Mcl-1 Antibody is Ion exchange chromatography purified.
Immunogen
Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the center of human Mcl-1. The immunogen is located within amino acids 110 - 160 of Mcl-1.
Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1S by Western blot at 0.5 to 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Mcl-1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
Mcl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
MCL-1 (MCL1)
(Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
Mcl-1 Antibody: Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.