TIMD4 antibody (AA 60-110)

Catalog No. ABIN6990640

Product Details anti-TIMD4 Antibody

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Binding Specificity: AA 60-110

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: At least four isoforms of TIM-4 are known to exist, this antibody will detect all four isoforms.

Purification: TIM-4 Antibody is affinity chromatography purified via peptide column.

Immunogen: TIM-4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4. The immunogen is located within amino acids 60 - 110 of TIM-4.

Isotype: IgG

Application Details

Application Notes: TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL and Immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human and mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TIM-4 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TIM-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TIMD4

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Alternative Name: TIM-4 (TIMD4 Products)

Synonyms: RGD1564516 antibody, MGC99322 antibody, TIMD4 antibody, B430010N18Rik antibody, TIM-4 antibody, Tim4 antibody, SMUCKLER antibody, TIM4 antibody, T-cell immunoglobulin and mucin domain containing 4 antibody, T-cell immunoglobulin and mucin domain containing 4 L homeolog antibody, T cell immunoglobulin and mucin domain containing 4 antibody, Timd4 antibody, TIMD4 antibody, timd4.L antibody

Background: TIM-4 Antibody: The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.

Molecular Weight: Observed: 45 kDa

Gene ID: 91937

NCBI Accession: NP_612388

UniProt: Q96H15

Pathways: Cancer Immune Checkpoints