SLUG antibody (AA 90-140)
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- Target See all SLUG products
- SLUG
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Binding Specificity
- AA 90-140
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLUG antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Specificity
- This Slug antibody is predicted to not cross-react with Snail.
- Purification
- Slug Antibody is affinity chromatography purified via peptide column.
- Immunogen
- Slug antibody was raised against a 13 amino acid synthetic peptide from near the center of human Slug. The immunogen is located within amino acids 90 - 140 of Slug.
- Isotype
- IgG
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- Application Notes
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Slug antibody can be used for detection of Slug by Western blot at 0.5 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL.
Antibody validated: Western Blot in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Slug Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- Slug antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Target
- SLUG
- Abstract
- SLUG Products
- Synonyms
- Slug antibody, Slugh antibody, Snail2 antibody, SLUG antibody, SLUGH antibody, SLUGH1 antibody, SNAIL2 antibody, WS2D antibody, snail family zinc finger 2 antibody, snail family transcriptional repressor 2 antibody, Snai2 antibody, SNAI2 antibody
- Background
- Slug Antibody: Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family.
- Molecular Weight
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Predicted: 29 kDa
Observed: 30 kDa - Gene ID
- 6591
- UniProt
- O43623
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