HUWE1 antibody (C-Term)

Catalog No. ABIN6990752

Product Details anti-HUWE1 Antibody

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Binding Specificity: AA 4250-4300, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUWE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: ARF-BP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ARF-BP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1. The immunogen is located within amino acids 4250 - 4300 of ARF-BP1.

Isotype: IgG

Application Details

Application Notes: ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ARF-BP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ARF-BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for HUWE1

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Alternative Name: ARF-BP1 (HUWE1 Products)

Synonyms: ARF-BP1 antibody, HECTH9 antibody, HSPC272 antibody, Ib772 antibody, LASU1 antibody, MULE antibody, URE-B1 antibody, UREB1 antibody, 5430439H10Rik antibody, AU041296 antibody, Arf-bp1 antibody, C430014N20Rik antibody, C80292 antibody, Gm1718 antibody, Mule antibody, Ureb1 antibody, HUWE1 antibody, si:dkey-242a8.2 antibody, Huwe1 antibody, mule antibody, ib772 antibody, lasu1 antibody, ureb1 antibody, hecth9 antibody, arf-bp1 antibody, hspc272 antibody, HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase antibody, HECT, UBA and WWE domain containing 1 antibody, E3 ubiquitin-protein ligase HUWE1 antibody, HUWE1 antibody, Huwe1 antibody, huwe1 antibody, LOC100123214 antibody, LOC100380641 antibody

Background: ARF-BP1 Antibody: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53, this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.

Gene ID: 10075

NCBI Accession: NP_113584

UniProt: Q7Z6Z7