Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
PTK7 Antibody is affinity chromatography purified via peptide column.
Immunogen
PTK7 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PTK7. The immunogen is located within amino acids 250 - 300 of PTK7.
PTK7 antibody can be used for detection of PTK7 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PTK7 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
PTK7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PTK7 Antibody: Protein-tyrosine kinases (PTKs) play important roles in regulating cell proliferation and differentiation during development. One member of the PTK family, PTK7, has been suggested to regulate the planar cell polarity (PCP) pathway in vertebrates and may play a role in neural convergent extension and neural tube closure. PTK7 has also been implicated in the development of cancer. Loss of PTK7 expression was seen in several melanoma cell lines and biopsies. Conversely, high-throughput analysis of acute myeloid leukemia samples showed an increased level of PTK7 expression compared to normal bone marrow and purified CD34+ cells. Multiple isoforms of PTK7 are known to exist.