AMBRA1 antibody (C-Term)

Catalog No. ABIN6990888

Product Details anti-AMBRA1 Antibody

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMBRA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Ambra1 Antibody is affinity chromatography purified via peptide column.

Immunogen: Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1. The immunogen is located within the last 50 amino acids of Ambra1.

Isotype: IgG

Application Details

Application Notes: Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Ambra1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Ambra1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for AMBRA1

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Alternative Name: Ambra1 (AMBRA1 Products)

Synonyms: 2310079H06Rik antibody, A130023A14 antibody, AA474864 antibody, AV021921 antibody, D030051N19Rik antibody, mKIAA1736 antibody, Nyw1 antibody, DCAF3 antibody, WDR94 antibody, autophagy and beclin 1 regulator 1 antibody, autophagy/beclin-1 regulator 1a antibody, autophagy/beclin 1 regulator 1 antibody, AMBRA1 antibody, ambra1a antibody, Ambra1 antibody

Background: Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

Gene ID: 55626

UniProt: Q9C0C7

Pathways: Autophagy