Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
At least two isoforms of integrin alpha 4 are known to exist.
Purification
Integrin alpha 4 Antibody is affinity chromatography purified via peptide column.
Immunogen
Integrin alpha 4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human Integrin alpha 4. The immunogen is located within amino acids 150 - 200 of Integrin alpha 4.
Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μ,g/mL. Antibody can also be used for immunoflourescence starting at 2 μ,g/mL.
Antibody validated: Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Integrin alpha 4 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
Integrin alpha 4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Integrin alpha 4 Antibody: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta1, the gut mucosal homing receptor for peripheral T cells.