alpha Tubulin antibody (N-Term)
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- Target See all alpha Tubulin (TUBA1) Antibodies
- alpha Tubulin (TUBA1)
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Binding Specificity
- AA 140-190, N-Term
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Reactivity
- Human, Mouse, Rat
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Host
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Chicken
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Clonality
- Polyclonal
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Conjugate
- This alpha Tubulin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purification
- Alpha-tubulin Antibody is affinity chromatography purified via peptide column.
- Immunogen
- Tubulin antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human Tubulin. The immunogen is located within amino acids 140 - 190 of Alpha-tubulin.
- Isotype
- IgY
- Top Product
- Discover our top product TUBA1 Primary Antibody
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- Application Notes
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Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 - 1 μ,g/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Alpha-tubulin Antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Target
- alpha Tubulin (TUBA1)
- Alternative Name
- Alpha-tubulin (TUBA1 Products)
- Synonyms
- Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody
- Background
- Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
- Gene ID
- 7278
- NCBI Accession
- NP_005992
- UniProt
- Q13748
- Pathways
- Microtubule Dynamics
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