APC2 antibody (AA 240-290)

Catalog No. ABIN6991322

Product Details anti-APC2 Antibody

Target: APC2 (APC Regulator of WNT Signaling Pathway 2 (APC2))

Binding Specificity: AA 240-290

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purification: APC2 Antibody is affinity chromatography purified via peptide column.

Immunogen: APC2 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC2. The immunogen is located within amino acids 240 - 290 of APC2.

Isotype: IgG

Application Details

Application Notes: APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: APC2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: APC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for APC2

Target: APC2 (APC Regulator of WNT Signaling Pathway 2 (APC2))

Alternative Name: APC2 (APC2 Products)

Synonyms: APCL antibody, AI852447 antibody, R75424 antibody, APC2, WNT signaling pathway regulator antibody, adenomatosis polyposis coli 2 antibody, APC2 antibody, Apc2 antibody

Background: APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.

Gene ID: 10297

UniProt: O95996

Pathways: WNT Signaling