Western Blotting (WB), Immunofluorescence (IF), ELISA
Specificity
At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. SARM antibody recognize the longest isoform.
Purification
SARM Antibody is affinity chromatography purified via peptide column.
Immunogen
SARM antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human SARM. The immunogen is located within amino acids 30 - 80 of SARM.
SARM1
Reactivity: Human, Mouse, Rat
WB, IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 555
Application Notes
SARM antibody can be used for detection of SARM by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SARM Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SARM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
SARM1
(Sterile alpha and TIR Motif Containing 1 (SARM1))
SARM Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SARM (SAM and ARM-containing protein), along with other molecules such as TIRP, TRIF, TIRAP, and MyD88, is thought to serve as an adaptor protein for the TLRs that allows for the activation of downstream kinases and NF-κ,B, and ultimately the expression of proteins involved in host defense. While SARM has not been conclusively shown to associate directly with TLRs, the presence of a Toll-interleukin-1 (TIR) domain in SARM is consistent with a role as a signaling molecule.