Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
At least two isoforms of UNG2 are known to exist, this antibody will only detect the longer isoform. UNG2 antibody is predicted to not cross-react with UNG1.
Purification
UNG2 Antibody is affinity chromatography purified via peptide column.
Immunogen
UNG2 antibody was raised against a 17 amino acid peptide near the amino terminus of human UNG2. The immunogen is located within the first 50 amino acids of UNG2.
UNG2 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promoters and alternative splicing. UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from DNA that arise through either misincorporation during replication or cytosine deamination. UNG2 can also be bound by the HIV-1 integrase and incorporated into the virion particle, suggesting that it is required to remove uracils from the viral genome. As the intrinsic antiviral protein APOBEC3G generates numerous uracils in the HIV genome during its replication, it may be that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome.