Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist, this antibody will detect all four.
Purification
FLI1 Antibody is affinity chromatography purified via peptide column.
Immunogen
FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1. The immunogen is located within the last 50 amino acids of FLI1 .
The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1). FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3). The FLI1 gene can undergo a t(11,22)(q24,q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4). Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5).