JAG1 antibody (C-Term)

Catalog No. ABIN6992092

Product Details anti-JAG1 Antibody

Target: JAG1 (Jagged 1 (JAG1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist, this antibody will detect both isoforms.

Purification: JAG1 antibody is affinity chromatography purified via peptide column.

Immunogen: JAG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAG1. The immunogen is located within the last 50 amino acids of JAG1.

Isotype: IgG

Application Details

Application Notes: JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: JAG1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: JAG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for JAG1

Target: JAG1 (Jagged 1 (JAG1))

Alternative Name: JAG1 (JAG1 Products)

Synonyms: AGS antibody, AHD antibody, AWS antibody, CD339 antibody, HJ1 antibody, JAGL1 antibody, ABE2 antibody, Gsfabe2 antibody, Htu antibody, Ozz antibody, Ser-1 antibody, JAG1 antibody, jag3 antibody, cb243 antibody, jag1 antibody, sb:cb243 antibody, serrateC antibody, jagged1 antibody, serrate antibody, serrate-1 antibody, x-serrate-1 antibody, jagged 1 antibody, jagged 1b antibody, jagged 1a antibody, jagged 1 L homeolog antibody, JAG1 antibody, Jag1 antibody, jag1 antibody, jag1b antibody, jag1a antibody, jag1.L antibody

Background: The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions (1). Ligands for Notch include JAG1, JAG2 and Delta. JAG1 is one of the DSL (Delta, Serrate, Lag2) transmembrane proteins that can activate Notch. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis (1,2). JAG1 is widely expressed in adult and fetal tissues and the mutation of the JAG1 gene is associated with Familial Tetrology of Fallot (3). JAG1 expression is associated with prostate cancer metastasis and is up-regulated in cervical squamous cell carcinoma (4,5).

Molecular Weight: Predicted: 134 kDa

Observed: 130 kDa

Gene ID: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Notch Signaling, Stem Cell Maintenance