KCNN4 antibody (3rd Extracellular Loop, Extracellular Loop) (Atto 488)

Catalog No. ABIN7043463

Product Details anti-KCNN4 Antibody (Atto 488)

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Binding Specificity: 3rd Extracellular Loop, Extracellular Loop

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNN4 antibody is conjugated to Atto 488

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Live Cell Imaging (LCI)

Characteristics: Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (ABIN7043462 and ABIN7044556)) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples. \nMouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody (#ABIN7043463) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified from cultured hybridoma medium.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Synthetic mapping to the 3rd extracellular loop of human KCNN4

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNN4

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Alternative Name: KCNN4 (KCa3.1, SK4) (KCNN4 Products)

Synonyms: KCa3.1 antibody, IK1 antibody, IKCA1 antibody, KCA4 antibody, SK4 antibody, hIKCa1 antibody, hKCa4 antibody, hSK4 antibody, rKCNN4c antibody, rSK4 antibody, SKCas antibody, mIKCa1 antibody, potassium calcium-activated channel subfamily N member 4 antibody, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 antibody, KCNN4 antibody, Kcnn4 antibody

Background: Alternative names: KCNN4 (KCa3.1, SK4), IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Gene ID: 3783

NCBI Accession: NM_002250

UniProt: O15554