SARS-CoV-2 Spike antibody
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- Target See all SARS-CoV-2 Spike Antibodies
- SARS-CoV-2 Spike
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Reactivity
- SARS-CoV-2
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SARS-CoV-2 Spike antibody is un-conjugated
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Application
- ELISA
- Purpose
- SARS-CoV-2 Whole Spike Protein Antibody
- Specificity
- Anti-SARS-CoV-2 Whole Spike antibody is directed against SARS Coronavirus 2 Spike (S) protein.
- Cross-Reactivity (Details)
- No reactivity was seen against SARS COV-2 Spike S1 subunit, SARS COV-2 Spike S1 RBD, SARS COV-2 loop, SARS COV-2 (N), MERS CoV. Cross reactivity with homologues from other sources has not been determined.
- Purification
- The product was purified from roller bottle culture by protein A chromatography. BLAST analysis was used to suggest reactivity with related Coronavirus proteins.
- Sterility
- Sterile filtered
- Immunogen
- This protein A purified antibody was produced by repeated immunizations with a purified SARS-CoV-2 S protein with His-tag, derived from the transfected human HEK293 cells.
- Clone
- 15D9-E2-C10
- Isotype
- IgG
- Top Product
- Discover our top product SARS-CoV-2 Spike Primary Antibody
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- Application Notes
- Anti-SARS-CoV-2 Whole Spike protein antibody has been tested for use in ELISA and shows reactivity to SARS COV-2 Whole Spike and COV-2 Spike (S2) protein. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Products
- Synonyms
- E2 antibody, E2 glycoprotein precursor antibody, Surface Glycoprotein antibody, S antibody
- Target Type
- Viral Protein
- Background
- SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein; and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development.
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