ATF2 antibody

Catalog No. ABIN7126255

Product Details anti-ATF2 Antibody

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene.

Cross-Reactivity (Details): Human. Predicted to react in mouse and rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ATF2 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Alternative Name: ATF2 (ATF2 Products)

Synonyms: CRE-BP1 antibody, CREB2 antibody, HB16 antibody, TREB7 antibody, CG12850 antibody, CG3749 antibody, dATF-2 antibody, DmelCG30420 antibody, CG30420 antibody, CHN1 antibody, Atf-2 antibody, CRE-BP antibody, Creb2 antibody, D130078H02Rik antibody, D18875 antibody, Tg(Gzma-Klra1)7Wum antibody, mXBP antibody, atf2-A antibody, atf2l antibody, activating transcription factor 2 antibody, Activating transcription factor-2 antibody, activating transcription factor 2 S homeolog antibody, ATF2 antibody, Atf-2 antibody, Atf2 antibody, atf2.S antibody, atf2 antibody

Background: HB16, CREB2, TREB7, CREB-2, CRE-BP1,ATF2
Cellular localisation: Nucleus

Molecular Weight: 54.5kDa

Gene ID: 1386, 592510

UniProt: P15336

Pathways: MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades