DNMT3A antibody

Catalog No. ABIN7126301

Product Details anti-DNMT3A Antibody

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Sadenosyl-L-methionin

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length DNMT3A protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Placenta.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3A (DNMT3A Products)

Synonyms: DNMT3A antibody, DNMT3A2 antibody, M.HsaIIIA antibody, MmuIIIA antibody, dnmt3a1 antibody, dnmt6 antibody, DNA methyltransferase 3 alpha antibody, DNA methyltransferase 3A antibody, DNA (cytosine-5-)-methyltransferase 3 alpha b antibody, DNMT3A antibody, Dnmt3a antibody, dnmt3a antibody, dnmt3ab antibody

Background: DNA methyltransferase 3 alpha, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT, DNMT3A2, M.HsaIIIA, MCMT, TBRS,DNMT3A / DNA Methyltransferase 3 Alpha
Cellular localisation: Nuclear

Molecular Weight: 102kDa

Gene ID: 1788, 154069

UniProt: Q16655